The regulation of tumor suppressor protein, p53, and estrogen receptor (ERα) by resveratrol in breast cancer cells
Julieta Saluzzo1,2, Kelly M. Hallman1,2, Katie Aleck1,2, Brigitte Dwyer1,2, Meghan Quigley1,2, Viktoria Mladenovik1,2, Amy E. Siebert1,2, and Sumi Dinda1,2
1 School of Health Sciences, Oakland University, Rochester, MI, USA
2 Prevention Research Center, Oakland University, Rochester, MI, USA
Correspondence:
Sumi Dinda, email:
Keywords: Breast cancer, tumor suppressors, p53, estrogen, antiestrogens
Received: October 29, 2015 Accepted: December 31, 2016 Published: January 04, 2017
Abstract
Resveratrol (RES) is a natural antioxidant found abundantly in grapes, peanuts, and berries, and is known to possess anti-tumorigenic properties. However, there is a noticeable lack of studies on the mechanistic effects of Resveratrol on tumor suppressors. Previous studies from our laboratory have shown the tumor suppressor protein p53 and estrogen receptor-alpha (ERα) to be possible molecular targets for RES. In this study, the anti-estrogenic effects of RES were analyzed on the expression of ERα and p53. The breast cancer cells grown in stripped serum were treated with 60 μM RES, as the optimum concentration based on data obtained from a concentration study using 1- 100 μM RES. Our studies indicate that RES caused a decrease in the levels of protein expression of p53 and ERα as compared to the control. Increasing concentrations of RES caused a four-fold decrease in cell number in comparison to estradiol. RES, in conjunction with ICI 182,780 (ICI), caused a down-regulation of both p53 and ERα as compared to the control. These observed effects on cell proliferation and regulation of both p53 and ERα by RES may lead to further understanding of the relationship between tumor suppressor proteins and steroid receptors in breast cancer cells.
